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1: W Gilbert Hartley in conversation with Philip C Robinson: a survey of light microscopy. 2: An illustrated lecture with slides and demonstrations by Gerard Turner of the Royal Microscopical Society. 3: P C...
Outlines the importance of the scanning electron microscope as a tool for biologists and the principles and practice of the preparation techniques in common use. Shows fixation, dehydration, critical point...
The history of microscopes and their uses and development to the present day.
Deals with the principles and constructional features of the transmission electron microscope, and in particular the imaging of dislocations. Develops around 100KeV and 1-MeV machines at Imperial College,...
After a brief historical review, it is shown from observations on living cells and by geometrical considerations that the double line appearance of most membranes, as well as the endoplasmic reticulum, the...
Deals with three basic techniques involved in microscopic imaging: bright field, dark field and phase contrast. Shows a simple microscope being developed using an optical benchand demonstrates the...
Negative staining a biological specimen with uranyl acetate. Floating a carbon carrier film on specimen suspension. Specimen particles are adsorbed to its underside. Washing the adsorbed particles by...
Describes the transmission electron microscope both as an instrument and as a diagnostic aid. Deals with the component parts of the microscope and some of its aberrations, follows on with routine preparative...
Demonstrates making a biological preparation by the adsorbtion method. Shows an electron micrograph of the flagella of the bacterium ‘Rhizobium lupini’ after shadowing with platinum.
Preparation of the specimen by glycerol treatment and centrifugation; freezing the specimen; preparing the freeze etching plant; course of fracturing, etching and shadowing the replica with platinum-iridium...
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