Electron-Microscopic Preparation Techniques: Freeze Etching

Synopsis: Preparation of the specimen by glycerol treatment and centrifugation; freezing the specimen; preparing the freeze etching plant; course of fracturing, etching and shadowing the replica with platinum-iridium alloy and carbon and cone shadowing with carbon; real-time and animation sequences; cleaning the replicas and mounting on carrier grids. Resulting freeze-etched replica of a longitudinally fractured cell of the green alga ‘Ankistrodesmus braunii’.
Language: English
Country: Germany
Medium: Video; Videocassette. VHS. col. 22 min.
Year of production: 1975
Availability: Sale; 1996 sale: £25.00 (+VAT +p&p)
Subjects: Biology
Keywords: biological specimens; electron microscopy; laboratory procedures; microscopy

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