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An introduction to the use of the binocular microscope.
Demonstrates the essential steps - fixation, embedding, sectioning, mounting and staining - required in the preparation of fresh animal tissue for viewing under the light microscope.
Demonstrates how to approach the microscopic examination of a fixed stain blood smear. The stain is the standard Giemsa stain.
Describes in detail the fluorescent antibody method for enumerating lymphocyte sub-populations defined by monoclonal antibodies. Ultra-violet microscopy and flow cytometry are used as a means of assessing...
Discusses the role of mitochondria in the production of ATP by the cell and shows how an oxygen electrode can be used to follow the uptake of oxygen by isolated mitochondria.
Covers description of the microscope, bright field, phase contrast, oil immersion, care of the microscope.
Looks at aspects of light microscope optics, illustrating the effects of the magnification achieved by different objective lenses, the roles of the condenser and iris diaphragm in improving resolution,...
First shows a large-scale polarising microscope, demonstrating the principles of optical mineralogy and includes animation about light polarisation and interference. Then shows the preparation of rock thin...
Discussion between Dr H Hillman and Dr Richard Gliddon. Begins with Dr Gliddon giving the history of microscopy up to the use of the electron microscope, thus providing some of the background information...
Gives a demonstration of fresh-tissue, fixation sectioning, staining and preparation for electron microscopy. Uses pancreatic acinar cell structures to illustrate the synthetic capacity of ribosome and rough...
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