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Video recording of the 2007 Croonian lecture given at the Royal Society on 3/5/2007. It has long been the goal of molecular biologists to design DNA binding proteins for the specific control of gene...
Examines techniques used in genetic analysis and modification and discusses their potential applications. Covers Polymerase chain reactions; Gel electrolysis; DNA sequencing and genetically modifed organisms...
In order to determine the order of nucleotides in DNA, it is necessary to perform DNA sequencing reactions. The ability to sequence double-stranded DNA, instead of having to prepare single-stranded DNA, is...
Documents the procedure of separating, or fractionating, reaction products of the dideoxy DNA sequencing reaction. By analysing the results of the electrophoresis, the order of nucleotides in DNA is...
Documents how radioactive nucleic acid probes are used to identify rare sequences of genomic DNA. Genomic DNA, fractionated by agarose gel electrophoresis, is transferred by capillary action onto nylon...
Separating different-sized proteins by electrophoresis is a very common method for characterising recombinant proteins. Shows the preparation of a basic denaturing discontinuous gel. In a separate...
Excerpts from the polymerase chain reaction PCR series.
The necessity to subclone fragments into appropriate vectors designed for sequencing can be substituted with the use of PCR. This saves considerable time and effort. Here, the plus and minus strands of...
Covers the three areas of transcription, protein synthesis, and mutations, using mainly computer-generated graphics. After an initial sequence showing the uses of proteins in living cells, the genetic code...
DNA fragments of less than 1 kilobase in length are separated through a 6% polyacrylamide gel by the use of an electrical field. The separated fragments are then visualised by ultraviolet light after...
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