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Introduces gene technology and demonstrates the laboratory techniques for performing gel electrophoresis and DNA transformation. The video reviews the structure of the DNA molecule and then explains and...
An introduction to recombinant DNA technology. Ssuitable for self-paced open learning or as part of a lecture course. Approximately 4 hours of teaching material. 1: An overview defining gene cloning,...
In this standard method to separate fragments of DNA, newly designed electrophoresis apparatus is used. The separation of fragments is visualised by ultraviolet light after staining the gels with ethidium...
Bacterial transformation, the process of cells taking up DNA, is important to molecular DNA and to grow large quantities of DNA. The procedures shown include making transformation-competent bacterial cells...
Excerpts from videos in the RECOMBINANT DNA series.
In order to determine the order of nucleotides in DNA, it is necessary to perform DNA sequencing reactions. The ability to sequence double-stranded DNA, instead of having to prepare single-stranded DNA, is...
The DNA ligation reaction causes two DNA molecules to be joined together. It is the basis of molecular cloning. Discusses theories and strategies of ligation and illustrates them using animation. These...
Documents the procedure of separating, or fractionating, reaction products of the dideoxy DNA sequencing reaction. By analysing the results of the electrophoresis, the order of nucleotides in DNA is...
Shows the process of isolating high molecular weight genomic DNA from bacteria. This involves lysis of bacterial cells, dialysis, alcohol precipitation, and spooling DNA onto a glass rod. Then DNA is...
Documents how radioactive nucleic acid probes are used to identify rare sequences of genomic DNA. Genomic DNA, fractionated by agarose gel electrophoresis, is transferred by capillary action onto nylon...
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