Dna Transformation

Synopsis: Introduces gene technology and demonstrates the laboratory techniques for performing gel electrophoresis and DNA transformation. The video reviews the structure of the DNA molecule and then explains and demonstrates: 1) ‘cutting and pasting’ - how a target sequence of DNA can be cut using restriction enzymes and combined with DNA from another organism using DNA licase to form recombinant DNA; how gene therapy can be used to replace defective genes. 2) Shows in detail the laboratory technique of gel electrophoresis which is used to separate specific segments of DNA and discusses how DNA analysis is used in biological research; 3) why bacteria are used for transferring DNA (smaller genomes, DNA contained in circular plasmids which provide useful vectors for delivering new genes to other cells); the use of marker genes; 4) DNA transformation in the laboratory - demonstrates an experiment to insert recombinant DNA into E.coli bacteria (genes enabling the bacteria to be more antibiotic-resistant and/or glow in the dark); 5) the importance of safety measures and sterilisation techniques when performing biological experiments in the laboratory.
Series: Science Lab Investigations Video Series
Language: English
Country: United States
Year of release: 2004
Year of production: 2003
Subjects: Biology
Keywords: gel electrophoresis; gene therapy; recombinant DNA techniques; DNA transformation

Name: Boulton-Hawker Films Ltd
Email: sales@boultonhawker.co.uk
Web: http://www.boultonhawker.co.uk/ External site opens in new window
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Address: Combs Tannery
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IP14 2EN
Notes: Distributor of over 400 DVDs and videos for use in teaching at GCSE level and above. Particularly strong in science-based subjects which the company used to produce themselves, but now sources from the USA, Canada and Australasia. Titles cover the subject areas of biology, business studies, careers, chemistry, child development & the family, citizenship, design & technology/food technology, English, geography/ecology, home economics, ICT, maths, media studies, PE/sport studies, physics and PSHE. Sale with free preview.

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