Learning on ScreenIntroduction to Recombinant Dna Technology: Purification and Manipulation of Dna
- Synopsis
- An introduction to recombinant DNA technology. Ssuitable for self-paced open learning or as part of a lecture course. Approximately 4 hours of teaching material.
1: An overview defining gene cloning, explaning general techniques used, and discussing applications within pharmacy and pharmacology.
2: Looks at experimental techniques used to purify the total cell DNA, plasmid DNA, bacteriophage DNA, single-stranded DNA, and mRNA. Also covers plasmid ampplification.
3: Introduces the range of enzymes used in gene cloning: nucleases, polymerases, modifying enzymes (alkaline phosphatase, polynucleotide kinase, terminal deoxynucleotidyl transferase), topoisomerases and ligases.
4: Looks at the types of restriction enzymes and discusses how restriction enzymes are used. In a workshop the student is asked to find and interpret results from restriction enzyme digestion using various restriction enzymes. The object is to find out where the restriction enzyme cutting sites are on a selection of example DN pieces. - Series
- Pccal: Pharmacy Consortium for Computer Aided Learning, Tltp Project
- Language
- English
- Country
- Great Britain
- Medium
- Multimedia; Computer program. Floppy disk. PC Windows version only.
- Year of production
- 1995
- Availability
- Sale
- Notes
- Available to UK higher education institutions.
Reviewed by Jonathan Heywood in ‘CTICM Update’ Vol 8 No 2 (12/97), p37. - Uses
- Undergraduate and postgraduate pharmacy.
- Subjects
- Biology; Medical sciences
- Keywords
- computer-assisted learning; pharmacy studies; recombinant DNA techniques; Teaching & Learning Technology Programme
Distribution Formats
- Type
- CD-ROM
Sections
- Title
- Gene cloning: an overview
- Synopsis
- 1: An overview defining gene cloning, explaning general techniques used, and discussing applications within pharmacy and pharmacology.
- Title
- Purification of DNA/RNA
- Synopsis
- 2: Looks at experimental techniques used to purify the total cell DNA, plasmid DNA, bacteriophage DNA, single-stranded DNA, and mRNA. Also covers plasmid ampplification.
- Title
- Manipulation of DNA
- Synopsis
- 3: Introduces the range of enzymes used in gene cloning: nucleases, polymerases, modifying enzymes (alkaline phosphatase, polynucleotide kinase, terminal deoxynucleotidyl transferase), topoisomerases and ligases.
- Title
- Restriction enzymes
- Synopsis
- 4: Looks at the types of restriction enzymes and discusses how restriction enzymes are used. In a workshop the student is asked to find and interpret results from restriction enzyme digestion using various restriction enzymes. The object is to find out wh
Production Company
Distributor
- Name
University of Bath, School of Pharmacy
- pccal@bath.ac.uk
- Web
- http://www.bath.ac.uk/PCCAL/ External site opens in new window
- Phone
- 01225 826826 ext 5110
- Address
- Claverton Down
Bath
BA2 7AY - Notes
- contact: Mr Keith Brown
Record Stats
This record has been viewed 687 times.
