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An introudction to the process by which DNA is transcribed and translated into proteins. Uses diagrams, animations, photographs and narratives to explain the one-gene-one polypeptide hypothesis, the...
CAL package in which the user is presented with a quantity of a newly isolated enzyme and asked to carry out an investigation to determine Vmax, Km, and their sensitivity to pH. Since the program contains...
Shows the process of isolating high molecular weight genomic DNA from bacteria. This involves lysis of bacterial cells, dialysis, alcohol precipitation, and spooling DNA onto a glass rod. Then DNA is...
DNA fragments often need to be recovered from the agarose to do enzymatic procedures, however, recovery from agarose can be quite poor. Here DNA is purified away from the agarose by an enzyme called...
These procedures are the basis for many recombinant DNA techniques. Here restriction enzymes are used to specifically cut DNA into smaller, analysable fragments. The results are analysed from photographs of...
Professor Patricia Hannah Clarke’s main research was on bacterial enzymes: their evolution and properties, and producing and purifying them for industrial use. She was also involved in developments in...
Enzymes are the workhorses of the cell and are very specific in their actions. This programme explores in detail the action of PFK, phospho fructose kinase, and explains specificity and catalysis. Uses...
Discusses how various organelles of a cell interact to form a working whole, using the chloroplast of plant cells to illustrate the process. Shows methods for isolating metabolically active chloroplasts from...
RNA can be difficult to isolate because of the ubiquitous presence of degrading enzymes. Here, whole cells are treated with guanidinium salt to rapidly deproteinise the RNA and denature ribonucleases that...
The ELISA (Enzyme Linked Immuno Sorbent Assay) test is used to measure the concentration of an antibody or an antigen in biological fluid. Shows in detail using graphics and laboratory sequences to explain...
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