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Explains how to sequence long stretches of DNA using phage or plasmid DNA as a vehicle, or vector, for the selection and analysis of individual fragments of the DNA of interest. The enzymatic method of...
Describes the two processes which form the basis of gene expression: the transcription of DNA and the translation of the resulting mRNA into protein. Current models for expressing prokaryotic and eukaryotic...
Focuses on the recent techniques for analysing genes and providing the information necessary to create a complete picture of the structure and function of DNA. Explains how gene probes are used to identify...
Shows how to construct DNA libraries in vectors, how to subsequently introduce them into bacteria and the screening methods used to isolate the DNA fragments of interest. Explains and compares the...
Discusses deletions where a section of DNA is removed; and substitutions, where the overall DNA length remains the same but the sequence changes. Includes use of deletion mutation to investigate...
Describes a new technique in which the DNA of interest is physically isolated by mechanical methods before cloning. Describes the procedures for isolating single chromosomes from a cell using microdissection...
Explains the complex chemical processes involved in the production of olignucleotides. Describes the phosphate diester, phosphate triester, phosphite triester and solid-phase methods of synthesis; HPLC...
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