Gene Analysis and Southern Blotting

Synopsis: Focuses on the recent techniques for analysing genes and providing the information necessary to create a complete picture of the structure and function of DNA. Explains how gene probes are used to identify DNA or RNA containing sequences of interest; how restriction enzymes cut up DNA into manageable sized fragments, and how the fragments are then separated by agarose gel electrophoresis. Illustrates how dot blotting, electroblotting and Southern blotting techniques are used to retrieve the DNA and immobilise it on thin membranes where it can be studied. Shows how Southern blotting is used to diagnose inherited genetic diseases.
Series: Techniques in Genetic Engineering, Series
Language: English
Country: Great Britain
Medium: Video; Videocassette. Standard formats. col. 25 min.
Year of production: 1984
Availability: Sale
Uses: 3rd-year and postgraduate life science students.*
Subjects: Biology
Keywords: biochemistry; DNA; genes; genetic disorders; laboratory procedures