Gene Analysis and Southern Blotting

Synopsis
Focuses on the recent techniques for analysing genes and providing the information necessary to create a complete picture of the structure and function of DNA. Explains how gene probes are used to identify DNA or RNA containing sequences of interest; how restriction enzymes cut up DNA into manageable sized fragments, and how the fragments are then separated by agarose gel electrophoresis. Illustrates how dot blotting, electroblotting and Southern blotting techniques are used to retrieve the DNA and immobilise it on thin membranes where it can be studied. Shows how Southern blotting is used to diagnose inherited genetic diseases.
Series
Techniques in Genetic Engineering, Series
Language
English
Country
Great Britain
Medium
Video; Videocassette. Standard formats. col. 25 min.
Year of production
1984
Availability
Sale
Uses
3rd-year and postgraduate life science students.*
Subjects
Biology
Keywords
biochemistry; DNA; genes; genetic disorders; laboratory procedures

Credits

Director
Mike Berry
Producer
Mark Bergin
Writer
Bob Williamson
Cast
Paul Vaughan 

Production Company

Name

Zoom Productions

Distributor

Name

Oxford University Press, Educational Marketing Department

Web
http://www.oup.co.uk External site opens in new window
Phone
01865 556767
Fax
01865 556646
Address
Walton Street
Oxford
OX2 6DP

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