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Gene Libraries

Synopsis: Shows how to construct DNA libraries in vectors, how to subsequently introduce them into bacteria and the screening methods used to isolate the DNA fragments of interest. Explains and compares the construction and use of the two main types of library - genomic and cDNA libraries. The section on genomic libraries covers the use of phage vectors, cosmid vectors, replica platings, radio-labelled probes, long-range mapping and cosmid walking techniques. Poly C tailing, the use of linkers, mRNA probes, chemically synthesised oligonucleotide probes and immunological identification techniques are featured in the section on cDNA libraries.
Series: Techniques in Genetic Engineering, Series
Language: English
Country: Great Britain
Medium: Video; Videocassette. Standard formats. col. 24 min.
Year of production: 1984
Availability: Sale
Uses: 2nd/3rd year undergraduates and M.Sc. students studying biochemistry, molecular biology and genetics.*
Subjects: Biology
Keywords: biochemistry; DNA; genes

Credits

Director

Producer

Writer

Cast

Production Company

Name: c/o Oxford University Press, Educational Marketing Department
Phone: 01865 556767 ext 4672
Fax: 01865 556646
Address: Walton Street
OXFORD
OX22 6DP
Notes: See: Oxford University Press, Educational Marketing Department

Name: Zoom Productions

Distributor

Name: Oxford University Press, Educational Marketing Department
Web: http://www.oup.co.uk External site opens in new window
Phone: 01865 556767
Fax: 01865 556646
Address: Walton Street
Oxford
OX2 6DP

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