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Dna Sequencing Using M13

Synopsis
Explains how to sequence long stretches of DNA using phage or plasmid DNA as a vehicle, or vector, for the selection and analysis of individual fragments of the DNA of interest. The enzymatic method of sequencing (called the chain terminator or dideoxy method) is featured and the bacteriophage M13 is used as a cloning vehicle. Illustrates the life cycle of M13, the M13 cloning method, sequencing with M13 and subsequent analysis.
Series
Techniques in Genetic Engineering, Series
Language
English
Country
Great Britain
Medium
Video; Videocassette. Standard formats. col. 29 min.
Year of production
1984
Availability
Sale
Uses
2nd-year biochemistry course in microbial genetics and molecular biology. 3rd-year biochemistry course in recombinant DNA technology. MSc biochemistry students.*
Subjects
Biology
Keywords
biochemistry; DNA sequencing

Credits

Director
Mike Berry
Producer
Mark Bergin
Writer
Stephen D M Brown
Cast
Paul Vaughan 

Production Company

Name

Zoom Productions

Distributor

Name

Oxford University Press, Educational Marketing Department

Web
http://www.oup.co.uk External site opens in new window
Phone
01865 556767
Fax
01865 556646
Address
Walton Street
Oxford
OX2 6DP

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