Dna Sequencing Using M13

Synopsis

Explains how to sequence long stretches of DNA using phage or plasmid DNA as a vehicle, or vector, for the selection and analysis of individual fragments of the DNA of interest. The enzymatic method of sequencing (called the chain terminator or dideoxy method) is featured and the bacteriophage M13 is used as a cloning vehicle. Illustrates the life cycle of M13, the M13 cloning method, sequencing with M13 and subsequent analysis.

Series

Techniques in Genetic Engineering, Series

Language

English

Country

Great Britain

Medium

Video; Videocassette. Standard formats. col. 29 min.

Year of production

1984

Availability

Sale

Uses

2nd-year biochemistry course in microbial genetics and molecular biology. 3rd-year biochemistry course in recombinant DNA technology. MSc biochemistry students.*

Subjects

Biology

Keywords

biochemistry; DNA sequencing

Credits

Director

Mike Berry

Producer

Mark Bergin

Writer

Stephen D M Brown

Cast

Paul Vaughan

Production Company

Name

Zoom Productions

Distributor

Name

Oxford University Press, Educational Marketing Department

Web

http://www.oup.co.uk External site opens in new window

Phone

01865 556767

Fax

01865 556646

Address

Walton Street
Oxford
OX2 6DP