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Examines techniques used in genetic analysis and modification and discusses their potential applications. Covers Polymerase chain reactions; Gel electrolysis; DNA sequencing and genetically modifed organisms...
Looks at the techniques of genetic engineering used to produce new combinations of genetic material not normally found in nature and the incorporation of this new genetic material into a host organism for...
Because of the exquisite sensitivity of PCR, contaminating DNA can be the major limiting factor in applying PCR techniques and should not be ignored. Considerations on how to avoid contamination were...
Excerpts from the polymerase chain reaction PCR series.
The necessity to subclone fragments into appropriate vectors designed for sequencing can be substituted with the use of PCR. This saves considerable time and effort. Here, the plus and minus strands of...
The exploration of scientific discovery has been facilitated by the polymerase chain reaction (PCR). The process of amplifying DNA of interest about one billion-fold by PCR is documented here. Precautions...
Procedures for preparing DNA for PCR amplification should be rapid and be performed with as few steps as possible to achieve a high yield of DNA and minimise the chances of contamination. The isolation of...
Examines the experimental use of techniques such as gel electrophoresis in DNA fingerprinting analysis. Discusses the mechanism of replication using a mixture of phase-contrast microscopy and accompanying...
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