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Negative staining a biological specimen with uranyl acetate. Floating a carbon carrier film on specimen suspension. Specimen particles are adsorbed to its underside. Washing the adsorbed particles by...
Demonstrates making a biological preparation by the adsorbtion method. Shows an electron micrograph of the flagella of the bacterium ‘Rhizobium lupini’ after shadowing with platinum.
Preparation of the specimen by glycerol treatment and centrifugation; freezing the specimen; preparing the freeze etching plant; course of fracturing, etching and shadowing the replica with platinum-iridium...
Shows making a carbon support film by sublimating graphite on to a mica sheet. Preparing the vacuum evaporation plant, deposition process, controlling the thickness of the coating by interference colour...
3Making a carbon-reinforced carrier film by the ‘flow out’ method, floating off the film on the water surface, judging its quality, coating support grids with film, removing the coated grids from the...
DNA and cytochrome C are spread on the surface of a salt solution. The spreading area is dabbed at random with filmed grids. In this way a thin layer of protein and DNA adheres to the grid. Shows contrast...
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