Working with Proteins (6 Parts)

Synopsis: Six videos looking at protein characterisation techniques, each with a tutorial on core tools and methodologies.
1: Cell lysis; differential centrifugation; ammonium sulphate precipiatation.
2: Ion-exchange chromatography; affinity chromatography; gel filtration chromatography.
3: SDS gels, isoelectric focusing; analytical ultracentrifugation.
4: Thin plate chromatography; enzyme activity assays.
5: Specific cleavage and overlap peptides; Edman degradation; MALD-TOF mass spectometry.
6: Protein crystallisation; X-ray diffraction; electron-density map interpretation; atomic modelling.
Series: Knowledge Now in Experimental Biology, Series
Language: English
Country: United States
Medium: Video; Videocassette. col. VHS. 6 x 30 min approx.
Year of production: 1997
Availability: Sale; 1998 sale: £59.95 (+VAT +p&p) each 1998 sale: £300.00 (+VAT +p&p) set
Documentation: Accompanying resource booklet with each part.
Uses: Undergraduates, postgraduates, industrial refresher courses.
Subjects: Biology
Keywords: biological assays; cells; chromatography; laboratory procedures; protein sequencing; proteins

Title: Fractionating a cell
Synopsis: 1: Cell lysis; differential centrifugation; ammonium sulphate precipiatation.
Title: Column chromatography
Synopsis: 2: Ion-exchange chromatography; affinity chromatography; gel filtration chromatography.
Title: Determining mass and shape
Synopsis: 3: SDS gels, isoelectric focusing; analytical ultracentrifugation.
Title: Activity assays
Synopsis: 4: Thin plate chromatography; enzyme activity assays.
Title: Sequencing a protein
Synopsis: 5: Specific cleavage and overlap peptides; Edman degradation; MALD-TOF mass spectometry.
Title: Solving crystal structures
Synopsis: 6: Protein crystallisation; X-ray diffraction; electron-density map interpretation; atomic modelling.

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