Large-Scale Preparation of Phage Dna

Synopsis
Lambda bacteriophage are used to clone large fragments of DNA. Here, phage are isolated by first infecting a liquid suspension of bacteria. During incubation the bacteria are lysed by the phage. The phage are then completely released from the bacteria with chloroform and purified by cesium chloride density centrifugation. Subsequently, the phage are extracted with phenol/chloroform extraction and dialysed with buffer.
Series
Recombinant Dna, Series
Language
English
Country
Great Britain
Medium
Video; Videocassette. VHS (PAL or NTSC). col. 29 min.
Year of production
1988
Availability
OUT OF DISTRIBUTION; 1994 sale: US$41.00 (inclusive)
Subjects
Biology
Keywords
bacteria; DNA; laboratory experiments; molecular biology

Distributor

Name

USA

Phone
+1 801 753 6911
Fax
+1 801 752 5615
Notes
Closed down 12/99

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