Immunoblotting

Synopsis: Explains why attempting to detect the presence of protein samples directly from an electrophoresis gel is impractical, due to diffusion of the protein bends over time and the fragility of the gel itself. Describes the principles of immunoblotting, transferring the information from the gel to a nitrocellulose membrane, first with diagrams and then in the laboratory. Also illustrates and demonstrates a method of making a single specific protein on the membrane visible using an antibody to that protein, then adding a second antibody with an enzyme attached that will produce a coloured product when a substrate is added.
Series: Shotlist, Series
Language: English
Country: Great Britain
Medium: Video; Videocassette. Standard formats. col. 13 min.
Year of production: 1997
Availability: Sale; 1999 sale: £55.00 (+VAT +p&p) VHS, non-higher education 1999 sale: £150.00 (+VAT +p&p) higher education - master tape for re-editing and copying 2000 sale: £35.00 (+VAT inc. p&p) VHS, higher education
Notes: Reviewed by Sally Wilks in ‘Journal of Biological Education’ Vol 32 No 2 (Summer 1998), p155.
Uses: Biomedical and biochemistry undergraduates; MSc immunology and related courses.
Subjects: Biology
Keywords: electrophoresis; immunoblotting; laboratory procedures; western blotting

Name: Educational Broadcasting Services Trust
Email: support@fenc.org.uk
Web: http://www.fenc.org.uk/ External site opens in new window
Phone: 01553 630828
Address: c/o fenc
The White House
8 Gayton Road
Ashwicken, Kings Lynn
Norfolk
PE32 1LS
Notes: EBST was set up in 1987, to spread specialist learning through broadcast and new media; it’s roots were in BBC and public service broadcasting. Now EBST Online is part of FENC (www.fenc.org.uk).

Name: Educational Broadcasting Services Trust
Address: No longer trading
Notes: Educational and training materials with an emphasis on maths, science and skills development, produced in collaboration with consortia of university and college academics and media departments under the auspices of the Educational Services Broadcasting Trust. Series include Maths for Engineers, Shotlist, and the Skillbank vocational skills training programmes. For sale on DVD or via download. Now ebst online is part of FENC. (www.fenc.org.uk). Now possibly part of Blended Learning Consortium.

Name: Leeds University Television
Contact: Sally Popplewell (Sales supervisor)
Email: mediaservices@leeds.ac.uk
Web: http://mediant.leeds.ac.uk/vtcatalogue/ External site opens in new window
Phone: 0113 343 2660
Fax: 0113 343 2669
Address: Media Services
University of Leeds
Leeds
LS2 9JT

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British Universities Film & Video Council