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In this standard method to separate fragments of DNA, newly designed electrophoresis apparatus is used. The separation of fragments is visualised by ultraviolet light after staining the gels with ethidium...
Bacterial transformation, the process of cells taking up DNA, is important to molecular DNA and to grow large quantities of DNA. The procedures shown include making transformation-competent bacterial cells...
Excerpts from videos in the RECOMBINANT DNA series.
In order to determine the order of nucleotides in DNA, it is necessary to perform DNA sequencing reactions. The ability to sequence double-stranded DNA, instead of having to prepare single-stranded DNA, is...
The DNA ligation reaction causes two DNA molecules to be joined together. It is the basis of molecular cloning. Discusses theories and strategies of ligation and illustrates them using animation. These...
Documents the procedure of separating, or fractionating, reaction products of the dideoxy DNA sequencing reaction. By analysing the results of the electrophoresis, the order of nucleotides in DNA is...
Shows the process of isolating high molecular weight genomic DNA from bacteria. This involves lysis of bacterial cells, dialysis, alcohol precipitation, and spooling DNA onto a glass rod. Then DNA is...
Documents how radioactive nucleic acid probes are used to identify rare sequences of genomic DNA. Genomic DNA, fractionated by agarose gel electrophoresis, is transferred by capillary action onto nylon...
Separating different-sized proteins by electrophoresis is a very common method for characterising recombinant proteins. Shows the preparation of a basic denaturing discontinuous gel. In a separate...
The need often arises to quickly isolate plasmid DNA transformed within bacteria away from genomic DNA and other cellular components. The procedure of lysing the bacteria, documented in the video, is called...
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