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Shows the process of isolating high molecular weight genomic DNA from bacteria. This involves lysis of bacterial cells, dialysis, alcohol precipitation, and spooling DNA onto a glass rod. Then DNA is...
Documents how radioactive nucleic acid probes are used to identify rare sequences of genomic DNA. Genomic DNA, fractionated by agarose gel electrophoresis, is transferred by capillary action onto nylon...
Separating different-sized proteins by electrophoresis is a very common method for characterising recombinant proteins. Shows the preparation of a basic denaturing discontinuous gel. In a separate...
The need often arises to quickly isolate plasmid DNA transformed within bacteria away from genomic DNA and other cellular components. The procedure of lysing the bacteria, documented in the video, is called...
Drawing on the expertise of molecular biologists, palaeontologists, embryologists and animal park designers, the programme builds a hypothetical ‘Jurassic Park’. Examines the real problems that lie in...
DNA fragments often need to be recovered from the agarose to do enzymatic procedures, however, recovery from agarose can be quite poor. Here DNA is purified away from the agarose by an enzyme called...
These procedures are the basis for many recombinant DNA techniques. Here restriction enzymes are used to specifically cut DNA into smaller, analysable fragments. The results are analysed from photographs of...
Because of the exquisite sensitivity of PCR, contaminating DNA can be the major limiting factor in applying PCR techniques and should not be ignored. Considerations on how to avoid contamination were...
Dr Barbara Pearse from the Laboratory of Molecular Biology in Cambridge describes her discovery of the protein clathrin, which plays an important role in this process. Other proteins, called receptors,...
Excerpts from the polymerase chain reaction PCR series.
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