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1: W Gilbert Hartley in conversation with Philip C Robinson: a survey of light microscopy. 2: An illustrated lecture with slides and demonstrations by Gerard Turner of the Royal Microscopical Society. 3: P C...
Outlines the importance of the scanning electron microscope as a tool for biologists and the principles and practice of the preparation techniques in common use. Shows fixation, dehydration, critical point...
Deals with the principles and constructional features of the transmission electron microscope, and in particular the imaging of dislocations. Develops around 100KeV and 1-MeV machines at Imperial College,...
Negative staining a biological specimen with uranyl acetate. Floating a carbon carrier film on specimen suspension. Specimen particles are adsorbed to its underside. Washing the adsorbed particles by...
Describes the transmission electron microscope both as an instrument and as a diagnostic aid. Deals with the component parts of the microscope and some of its aberrations, follows on with routine preparative...
Demonstrates making a biological preparation by the adsorbtion method. Shows an electron micrograph of the flagella of the bacterium ‘Rhizobium lupini’ after shadowing with platinum.
Preparation of the specimen by glycerol treatment and centrifugation; freezing the specimen; preparing the freeze etching plant; course of fracturing, etching and shadowing the replica with platinum-iridium...
Shows making a carbon support film by sublimating graphite on to a mica sheet. Preparing the vacuum evaporation plant, deposition process, controlling the thickness of the coating by interference colour...
3Making a carbon-reinforced carrier film by the ‘flow out’ method, floating off the film on the water surface, judging its quality, coating support grids with film, removing the coated grids from the...
DNA and cytochrome C are spread on the surface of a salt solution. The spreading area is dabbed at random with filmed grids. In this way a thin layer of protein and DNA adheres to the grid. Shows contrast...
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