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Sterile cell culture techniques are shown by documenting actual experiments, showing the maintenance and passage of cultures. The investigators use techniques such as disinfecting instruments and work areas,...
An important part of cell culture is the ability to cryopreserve or freeze cells as a method of storing them. Shows in an actual experiment the method and principles of cryopreserving cells using DMSO with...
This contains excerpts from the videotapes (601-605) and gives a good representation of the programmes.
Video microscope images are used to characterise commonly used cells. Cells of different morphologies, shapes, sizes, growth patterns and organelle definitions are seen through a phase contrast microscope....
Shows the proper method for quantifying viable cells using a hemocytometer in an actual experiment. Viable cells are distinguished from unhealthy cells with and without using trypan blue exclusion. Also...
Many commonly used cell lines require a solid matrix on which to grow, such as tissue culture plastics. Once they take up all the space on the surface of the plastic, they need to be removed with...
The experimental basis of chemistry, starting with the action of heat on substances and continues with combustion, oxidation and the properties of oxides of metals and non-metals. Shows compounds including...
Procedures for preparing DNA for PCR amplification should be rapid and be performed with as few steps as possible to achieve a high yield of DNA and minimise the chances of contamination. The isolation of...
Two video sequences showing a series of demonstrations of experimental techniques which include: continuous extractions; flash chromatography; reduced-pressure distillation; small-scale recrystallisation and...
Illustrates the apparatus needed for tissue culture; how to prepare media; how to maintain cells in continuous culture, both as a monolayer and in suspension; how to recognise contamination of cultures and...
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