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19: Using a flame photometer, using an atomic absorption spectrometer, colorimetric analysis.
Simulation of an experiment on freshly killed rats to study the effect of starvation on induction of gluconeogenic enzymes, and includes various procedures such as the weighing of sections and their...
Excerpts from videos in the RECOMBINANT DNA series.
Because of the exquisite sensitivity of PCR, contaminating DNA can be the major limiting factor in applying PCR techniques and should not be ignored. Considerations on how to avoid contamination were...
Excerpts from the polymerase chain reaction PCR series.
RNA can be difficult to isolate because of the ubiquitous presence of degrading enzymes. Here, whole cells are treated with guanidinium salt to rapidly deproteinise the RNA and denature ribonucleases that...
Lambda bacteriophage are used to clone large fragments of DNA. Here, phage are isolated by first infecting a liquid suspension of bacteria. During incubation the bacteria are lysed by the phage. The phage...
Bacterial plasmids are used to clone DNA. Here, large amounts of bacteria are cultured containing the plasmid. The numbers of plasmids in the bacteria are then amplified by the addition of chloramphenicol....
Tissue culture techniques are valuable on humanitarian grounds in that they can greatly reduce the use of laboratory animals. In certain cases they are also attractive for reasons of reduced cost or greater...
Designed to support A-level physics courses, each programme enables students to make observations and readings as if they were performing experiments in the laboratory. Titles of individual programmes are:...
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