Fertilization of Mammalian Eggs in the Laboratory

Synopsis

'In vitro’ fertilisation has become a major tool in experimental biology and of considerable importance for assisting reproduction in infertile couples. Gives a step-by-step account of IVF in the laboratory mouse: 1) hormonal stimulation of ovarian follicle development; 2) preparation of culture media; 3) isolation of eggs and sperm and their combination in vitro; 4) culture of embryos through preimplantation stages; 5) transfer of embryos to pseudopregnant host mothers followed by the birth of pups. Provides details of the techniques at each step, and explains where relevant, the developmental biology.

Language

English

Country

Great Britain

Medium

Video; Videocassette. Standard formats. col. 34 min.

Year of production

1990

Availability

Sale

Uses

Medical students, intermediate level undergraduate in physiology and developmental biology.

Subjects

Biology; Medical sciences

Keywords

culturing techniques; fertilisation; mouse; tissue cultures

Credits

Producer

R G Gosden

Contributor

D F Bailey

Distributor

Name

University of Edinburgh Medical School, Department of Physiology