Cell Structure and Energy Production (4 Parts)

Synopsis: Discusses the role of mitochondria in the production of ATP by the cell and shows how an oxygen electrode can be used to follow the uptake of oxygen by isolated mitochondria.
Series: Biochemical Basis of Biology, Series
Language: English
Country: Great Britain
Medium: Video; Videocassette. Standard formats. col. 60 min.
Year of production: 1986
Availability: out of distribution; 1995 sale: £46.00 (+VAT +p&p)
Documentation: Accompanying teacher’s manual.
Uses: A-level courses and 1st-year undergraduate biology.*
Subjects: Biology
Keywords: cells; chloroplasts; electron microscopy; laboratory procedures; microscopy; mitochondria; photosynthesis

Title: Electron microscope, The
Synopsis: Part 1 (15 min): Introduces the electron microscope by means of live action shots and graphics, and shows how specimens are prepared, sectioned and stained. Electron micrographs are used throughout to illustrate the structure of cell organelles, bacteria
Title: Subcellular fractionation
Synopsis: Part 2 (15 min): Discusses the difficulties encountered in isolating different cell organelles and shows how mitochondria can be isolated and purified from a rat liver using the technique of sub-cellular fractionation. Demonstrates several different types
Title: Micochondrial respiration: the oxygen electrode
Synopsis: Part 3 (15 min): By using an oxygen electrode, the rate of oxygen uptake by respiring mitochondria can be followed. Shows how an an oxygen electrode is assembled and used, and the results that are obtained, using mitochondria isolated by sub-cellular frac
Title: Light reaction, The
Synopsis: Part 4 (15 min): Shows the procedure for isolating chloroplasts from spinach leaves and demonstrates how the light reaction of photosynthesis can be studied in the laboratory.

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